Difference between revisions of "Part:BBa K2936003"
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Since the accumulation of formic acid would affect the growth of the strain, in order to further optimize the degradation efficiency of FDS-2 for formaldehyde, we found that LBFDH existing in L. buchneri could degrade formate into CO2. By introducing the gene controlling LBFDH expression, hoping to reduce the accumulation of formic acid in bacteria and improve the degradation efficiency of formate. ( Degradation principle is shown in Figure 1, and the plasmid map is shown in Figure2) | Since the accumulation of formic acid would affect the growth of the strain, in order to further optimize the degradation efficiency of FDS-2 for formaldehyde, we found that LBFDH existing in L. buchneri could degrade formate into CO2. By introducing the gene controlling LBFDH expression, hoping to reduce the accumulation of formic acid in bacteria and improve the degradation efficiency of formate. ( Degradation principle is shown in Figure 1, and the plasmid map is shown in Figure2) | ||
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Revision as of 20:45, 20 October 2019
S- glutathione hydrolase(Regulated by flhA gene)
This is the coding sequence of the S- glutathione hydrolase. The S- glutathione hydrolase oxidizes GSCHO to formic acid.
Usage and Biology
Since the accumulation of formic acid would affect the growth of the strain, in order to further optimize the degradation efficiency of FDS-2 for formaldehyde, we found that LBFDH existing in L. buchneri could degrade formate into CO2. By introducing the gene controlling LBFDH expression, hoping to reduce the accumulation of formic acid in bacteria and improve the degradation efficiency of formate. ( Degradation principle is shown in Figure 1, and the plasmid map is shown in Figure2)
