Difference between revisions of "Part:BBa K2978202"

Line 7: Line 7:
 
The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.
 
The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.
  
This specific terminator is descripted in the next link  
+
This specific terminator is descripted in the next link:
 
https://parts.igem.org/Part:BBa_B0015
 
https://parts.igem.org/Part:BBa_B0015
  

Revision as of 20:11, 20 October 2019


B0015 Terminators Measurement in Lactobacillus spp

This part is the negative control for the efficiency terminators assay, where we tested three different sequences for Lactobacillus spp K2978201 K2978202 K2978203 K2978204 and K2978205.

The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.

This specific terminator is descripted in the next link: https://parts.igem.org/Part:BBa_B0015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]