Difference between revisions of "Part:BBa K2978211"

Revision as of 19:55, 20 October 2019

The in silico sequence was obtained from the Duong et al (2010) where they tested this Rho-independent terminator and the K2978212 terminator.
This terminator determined with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-23.5 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong et al 2010).
The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
The composite part of this sequence is present in K2978203

Terminator for Lactobacillus spp

Secondary Structure

Assembly Compatibility:

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 ===Description===
 * The <i>in silico</i> sequence was obtained from the Duong <i>et al</i> (2010) where they tested this Rho-independent terminator and the [[Part:BBa_K2978212| K2978212]] terminator.
-*This terminator was identified with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-24.4 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong <i> et al</i> 2010).
+*This terminator determined with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-23.5 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong <i> et al</i> 2010).
 *The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
 * The composite part of this sequence is present in [[Part:BBa_K2978203| K2978203]]