Difference between revisions of "Part:BBa K3081104"

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This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.
 
This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.
https://2019.igem.org/wiki/images/9/99/T--Peking--crispi-1.png
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https://2019.igem.org/wiki/images/2/27/T--Peking--pbad.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:00, 20 October 2019


scaffold of sgRNA in CRISPR/Cas system

This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold. T--Peking--pbad.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]