Difference between revisions of "Part:BBa K3113302"
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<partinfo>BBa_K3113302 short</partinfo> | <partinfo>BBa_K3113302 short</partinfo> | ||
| − | + | This plasmid codes for the coat protein of gag fused to a split luciferase fused to an RNA binding protein. This construct forms virus-like particles which can be detected via a split luciferase assay. Through the RNA binding protein, the vesicles can be loaded with RNA. | |
| + | ===Usage=== | ||
| + | |||
| + | pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301<ref>https://parts.igem.org/Part:BBa_K3113301</ref> and BBa_K3113203<ref>https://parts.igem.org/Part:BBa_K3113303</ref>). | ||
| − | + | ===Biology=== | |
| − | === | + | |
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Revision as of 18:53, 20 October 2019
pCAG_Gag-HiBiT-L7Ae
This plasmid codes for the coat protein of gag fused to a split luciferase fused to an RNA binding protein. This construct forms virus-like particles which can be detected via a split luciferase assay. Through the RNA binding protein, the vesicles can be loaded with RNA.
Usage
pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301[1] and BBa_K3113203[2]).
Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3019
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2319