Difference between revisions of "Part:BBa K3317067"
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===Usage and Biology===
 
===Usage and Biology===
  
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The primary objective was to perform an "in silico" design to reduce the range of operation of previously reported thermometers (28-37 ° C), obtaining a more precise range and allowing greater control of the system.  
+
The primary objective was to perform an <i>in silico</i> design to reduce the range of operation of previously reported thermometers (28-37° C), obtaining a more precise range and allowing greater control of the system.  
  
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Tests were performed in conjunction with the (<partinfo>K1140006</partinfo>) thermometer from 25 to 40 ° C, measuring at 586/610 nm (excitation / emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum.
+
Tests were performed in conjunction with the (<partinfo>K1140006</partinfo>) thermometer from 25 to 40° C, measuring at 586/610 nm (excitation/emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum.
  
  

Revision as of 04:07, 21 October 2019


37 C inducible RNA thermometer

RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (BBa_K1140006)

Usage and Biology

The primary objective was to perform an in silico design to reduce the range of operation of previously reported thermometers (28-37° C), obtaining a more precise range and allowing greater control of the system.

Tests were performed in conjunction with the (BBa_K1140006) thermometer from 25 to 40° C, measuring at 586/610 nm (excitation/emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters