Difference between revisions of "Part:BBa K3317067"
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RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (<partinfo>K1140006</partinfo>) | RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (<partinfo>K1140006</partinfo>) | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Tests were performed in conjunction with the (<partinfo>K1140006</partinfo>) thermometer from 25 to 40 ° C, measuring at 586/610 nm (excitation / emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum. | Tests were performed in conjunction with the (<partinfo>K1140006</partinfo>) thermometer from 25 to 40 ° C, measuring at 586/610 nm (excitation / emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3317067 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3317067 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3317067 parameters</partinfo> | <partinfo>BBa_K3317067 parameters</partinfo> | ||
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Revision as of 17:02, 20 October 2019
37 C inducible RNA thermometer
RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (BBa_K1140006)
Usage and Biology
The primary objective was to perform an "in silico" design to reduce the range of operation of previously reported thermometers (28-37 ° C), obtaining a more precise range and allowing greater control of the system.
Tests were performed in conjunction with the (BBa_K1140006) thermometer from 25 to 40 ° C, measuring at 586/610 nm (excitation / emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]