Difference between revisions of "Part:BBa K3147005:Design"
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The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G [[Part:BBa_ J18918]] with \ indicating cleaved peptide binding. | The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G [[Part:BBa_ J18918]] with \ indicating cleaved peptide binding. | ||
− | The Terminator used is a double terminator [[Part: | + | The Terminator used is a double terminator [[Part:BBa_B0015]]. |
Latest revision as of 16:35, 20 October 2019
TEV protease with TEV-cleavable maltose binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 379
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Note
The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.
The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G Part:BBa_ J18918 with \ indicating cleaved peptide binding.
The Terminator used is a double terminator Part:BBa_B0015.