Difference between revisions of "Part:BBa K2924039"

Revision as of 18:46, 20 October 2019

AOX1 methanol-inducible promoter

Methanol is processed in P. pastoris by an alcohol oxidases (AOX) converting it to formaldehyde, which are coded by AOX genes¹. The AOX1 promotor is highly induced by methanol, repressed in the presence of other carbon sources like glucose, and therefore genes under the control of the promotor are highly expressed in the presence of methanol and can be easily regulated^2,3. Methylotrophic promotors are among the strongest and strictly regulated yeast promotors, which makes them suitable for controlled strong expression of target proteins after methanol induction⁴. The AOX1 promotor can produce a high yield of recombinant proteins up to 30 % of the total mass of cell or up to 22 g/L intercellular protein in P. pastoris⁵.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-Methanol is processed in P. pastoris by an alcohol oxidases (AOX) converting it to formaldehyde, which are coded by AOX genes (Tschopp et al., 1987). The AOX1 promotor is highly induced by methanol, repressed in the presence of other carbon sources like glucose, and therefore genes under the control of the promotor are highly expressed in the presence of methanol and can be easily regulated (Tschopp et al., 1987; Daly and Hearn, 2005). Methylotrophic promotors are among the strongest and strictly regulated yeast promotors, which makes them suitable for controlled strong expression of target proteins after methanol induction (Demain and Vaishnav, 2011). The AOX1 promotor can produce a high yield of recombinant proteins up to 30 % of the total mass of cell or up to 22 g/L intercellular protein in P. pastoris (Romanos et al., 1992; Vogl et al., 2013).
+Methanol is processed in <I>P. pastoris</I> by an alcohol oxidases (AOX) converting it to formaldehyde, which are coded by AOX genes<sup>1</sup>. The AOX1 promotor is highly induced by methanol, repressed in the presence of other carbon sources like glucose, and therefore genes under the control of the promotor are highly expressed in the presence of methanol and can be easily regulated<sup>2,3</sup>. Methylotrophic promotors are among the strongest and strictly regulated yeast promotors, which makes them suitable for controlled strong expression of target proteins after methanol induction<sup>4</sup>. The AOX1 promotor can produce a high yield of recombinant proteins up to 30 % of the total mass of cell or up to 22 g/L intercellular protein in <I>P. pastoris</I><sup>5</sup>.
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