Difference between revisions of "Part:BBa K3286003"
 
Line 3: Line 3:
 
<partinfo>BBa_K3286003 short</partinfo>
 
<partinfo>BBa_K3286003 short</partinfo>
  
−
Expression cassette of the sgRNA for Cas9 of <i>Streptococcus pyogenes</i> with non-targeting (NT) spacer [[Part:BBa_K3286001]] and rho-independent terminator [[Part:BBa_K3286002]] under control of the strong constitutive Anderson promoter J23109 [[Part:BBa_J23119]].  
+
Expression module of the sgRNA scaffold for Cas9 of <i>Streptococcus pyogenes</i> with non-targeting (NT) spacer [[Part:BBa_K3286001]] and rho-independent terminator [[Part:BBa_K3286002]] under control of the strong constitutive Anderson promoter J23109 [[Part:BBa_J23119]].  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:22, 21 October 2019


sgRNA expression module

Expression module of the sgRNA scaffold for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer Part:BBa_K3286001 and rho-independent terminator Part:BBa_K3286002 under control of the strong constitutive Anderson promoter J23109 Part:BBa_J23119.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]