Difference between revisions of "Part:BBa K2984018"
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Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432. | Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432. | ||
+ | </li> | ||
+ | <li>Stevens, D. R., Purton, S., & Rochaix, J.-D. (1996). The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas. Molecular and General Genetics MGG, 251(1), 23–30. https://doi.org/10.1007/BF02174340 | ||
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</ol> | </ol> |
Latest revision as of 21:03, 21 October 2019
RbcS2 - Terminator B5-C1
This part is a part of the Chlamy-HUB-Collection. To terminate the transcription of a gene a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).
This part was designed to be used with the MoClo standard and has B5-C1 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 258
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
- Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.
- Stevens, D. R., Purton, S., & Rochaix, J.-D. (1996). The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas. Molecular and General Genetics MGG, 251(1), 23–30. https://doi.org/10.1007/BF02174340