Difference between revisions of "Part:BBa K2922040"

Revision as of 13:23, 20 October 2019

Caveolin-1 coding region

Summary

This part contains the coding region of caveolin-1 gene. Caveolae are endocytic pits located in mammalian cell surfaces where they play indispensible roles in endocytosis.

Figure 1. Schematic diagram of action principle for CAV1

It has been shown that the formation of heterologous caveolae (h-caveolae) can be observed in the cytoplasm of E. coli after the heterologous expression of caveolin-1. Thus, we chose CAV1 to perform endocytosis in E.coli.

Identification

After receiving the synthesized Part DNA, restriction digest identification was done to certify the plasmid is correct, and and the experimental results were shown in Fig. 1. A separate fragment is 537 bp.

<style="width:40%"> Fig 2.DNA gel electrophoresis of restriction digest products of DH-Cav1-pUC57 (Xbal I & Pst I sites)

Reference

1. J. Shin et al., Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm. Enzyme Microb Technol 79-80, 55-62 (2015).
2. J. Shin et al., Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids. ACS Synthetic Biology 8, 1055-1066 (2019).

Sequence and Features