Difference between revisions of "Part:BBa K3081014"
| Line 5: | Line 5: | ||
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the integration host factor (IHF) binding site on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. | This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the integration host factor (IHF) binding site on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. | ||
| + | <H1>Experiment</H1> | ||
For more detailed information, see <partinfo>BBa_K3081058</partinfo> | For more detailed information, see <partinfo>BBa_K3081058</partinfo> | ||
Revision as of 18:28, 21 October 2019
pBAD-dCas9-J23119-IHF
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the integration host factor (IHF) binding site on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation.
Experiment
For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1470
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961