Difference between revisions of "Part:BBa K2970000"
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<partinfo>BBa_K2970000 short</partinfo>
 
<partinfo>BBa_K2970000 short</partinfo>
  
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Trigger 1 forms together with Trigger 2 (<partinfo>BBa_K2970001</partinfo>) a complex due to complementary regions. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur. The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
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Trigger 1 forms together with Trigger 2 (<partinfo>BBa_K2970001</partinfo>) a complex due to complementary regions, as shown in Figure 1. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur (Figure 2). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
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<figure>
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<img width="70%" src="https://2019.igem.org/wiki/images/4/4f/T--Hamburg--Part_Figure3.jpg">
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<figcaption>
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<b>Figure 1: </b>Trigger complex formation
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<html>
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<figure>
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<img width="70%" src="https://2019.igem.org/wiki/images/4/49/T--Hamburg--Part_Figure6.jpg">
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<figcaption>
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<b>Figure 1: </b>Trigger complex formation
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To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970003</partinfo>).  
 
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970003</partinfo>).  
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After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.
 
After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.
  

Revision as of 08:41, 21 October 2019


Trigger 1

Trigger 1 forms together with Trigger 2 (BBa_K2970001) a complex due to complementary regions, as shown in Figure 1. This complex can open a gate (BBa_K2970002) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur (Figure 2). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.

Figure 1: Trigger complex formation
Figure 1: Trigger complex formation

To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (BBa_K2970003). After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]