Difference between revisions of "Part:BBa I712004:Experience"
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===Applications of BBa_I712004=== | ===Applications of BBa_I712004=== | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708. | We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708. | ||
[[File:2004_figure1.jpg|720px]] | [[File:2004_figure1.jpg|720px]] | ||
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Fig.2 Transfection cells observed under a fluorescence microscope | Fig.2 Transfection cells observed under a fluorescence microscope | ||
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Revision as of 12:28, 20 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I712004
Usage and Biology
User Reviews
We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
Fig.1 The plasmid we used for transfection.
Fig.2 Transfection cells observed under a fluorescence microscope UNIQc5c9913d7645411b-partinfo-00000000-QINU UNIQc5c9913d7645411b-partinfo-00000001-QINU