Difference between revisions of "Part:BBa K2970009"
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<partinfo>BBa_K2970009 short</partinfo> | <partinfo>BBa_K2970009 short</partinfo> | ||
| − | Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone <partinfo>pSB1C3- | + | Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone <partinfo>pSB1C3-BBa_E0240</partinfo> for characterization. |
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Revision as of 12:16, 20 October 2019
J23100 Test Composition with RiboJ
Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone No part name specified with partinfo tag. for characterization.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 789