Difference between revisions of "Part:BBa K2970002"
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<partinfo>BBa_K2970002 short</partinfo> | <partinfo>BBa_K2970002 short</partinfo> | ||
− | This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (<partinfo>BBa_K2970000</partinfo> and <partinfo>BBa_K2970001</partinfo>). The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence. | + | This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (<partinfo>BBa_K2970000</partinfo> and <partinfo>BBa_K2970001</partinfo>). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence. |
To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (<partinfo>BBa_K2970006</partinfo>). | To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (<partinfo>BBa_K2970006</partinfo>). |
Revision as of 12:24, 20 October 2019
Gate
This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (BBa_K2970000 and BBa_K2970001). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (BBa_K2970006).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]