Difference between revisions of "Part:BBa K3040005"
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<p style="color:gray; font-size:13px; text-align:center;">This picture shows the consensus sequence in E coli and some mutant sequence of this region.</p> | <p style="color:gray; font-size:13px; text-align:center;">This picture shows the consensus sequence in E coli and some mutant sequence of this region.</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:44, 20 October 2019
Native pFadD promoter regulating downstream RFP
Description
It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA (BBa_K817002) has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA by change consensus sequence of -10 and -35 region. According to the previous research, there is improvement in expression for pFadBA with consensus sequence.
Mechanism
Some study shows that in E. coli, there is sequence called consensus sequence which is universal and change the expression level of downstream protein. Consensus sequence is existing in -10 and -35 region, so it is relative with gene expression.
This picture shows the consensus sequence in E coli and some mutant sequence of this region.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 755
Illegal AgeI site found at 867 - 1000COMPATIBLE WITH RFC[1000]