Difference between revisions of "Part:BBa K3040005"
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===Description===
 
===Description===
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It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region.
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It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA (BBa_K817002) has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA by change consensus sequence of -10 and -35 region.
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According to the previous research [1], there is improvement in expression for pFadBA with consensus sequence.
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According to the previous research, there is improvement in expression for pFadBA with consensus sequence.  
  
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===Mechanism===
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Some study shows that in E. coli, there is sequence called consensus sequence which is universal and change the expression level of downstream protein. Consensus sequence is existing in -10 and -35 region, so it is relative with gene expression.
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<br>
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<html>
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      <div style="margin:auto;">
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      <img src="https://static.igem.org/mediawiki/parts/0/05/T--NTHU_Taiwan--liya1.png" style="width:70%; align:center;">
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      <p style="color:gray; font-size:13px; text-align:center;">This picture shows the consensus sequence in E coli and some mutant sequence of this region.</p>
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</div>
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</html>
  
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<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3040013 parameters</partinfo>
 
<partinfo>BBa_K3040013 parameters</partinfo>
 
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Revision as of 11:43, 20 October 2019

Native pFadD promoter regulating downstream RFP

Description

It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA (BBa_K817002) has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA by change consensus sequence of -10 and -35 region. According to the previous research, there is improvement in expression for pFadBA with consensus sequence.

Mechanism

Some study shows that in E. coli, there is sequence called consensus sequence which is universal and change the expression level of downstream protein. Consensus sequence is existing in -10 and -35 region, so it is relative with gene expression.

This picture shows the consensus sequence in E coli and some mutant sequence of this region.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 755
    Illegal AgeI site found at 867
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters