Difference between revisions of "Part:BBa K3245006"
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<h2>Usage and biology:</h2> | <h2>Usage and biology:</h2> | ||
<p>This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS100). This part features low leakage when luxpR is not induced, approximately one fifth of that of BBa_K234005. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245006 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | <p>This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS100). This part features low leakage when luxpR is not induced, approximately one fifth of that of BBa_K234005. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245006 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | ||
Revision as of 10:14, 20 October 2019
Usage and biology:
This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS100). This part features low leakage when luxpR is not induced, approximately one fifth of that of BBa_K234005. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245006 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect.