Difference between revisions of "Part:BBa K3245004"
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<h2>Usage and biology:</h2> | <h2>Usage and biology:</h2> | ||
<p>This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS106). This part hasn’t been thoroughly characterized yet and further characterization is welcomed. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245004 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | <p>This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS106). This part hasn’t been thoroughly characterized yet and further characterization is welcomed. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245004 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | ||
Latest revision as of 10:14, 20 October 2019
Usage and biology:
This part is used to express tetR under the control of our engineered quorum sensing promoter luxpR(HS106). This part hasn’t been thoroughly characterized yet and further characterization is welcomed. To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245004 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect.