Difference between revisions of "Part:BBa K2913021"
(→result) |
(→result) |
||
| Line 13: | Line 13: | ||
We added gradient of 5-FC culturing the bacteria and monitor their growth. | We added gradient of 5-FC culturing the bacteria and monitor their growth. | ||
| − | [[Image:T--NEFU_China--parts--CD1.png|600px|thumb| | + | [[Image:T--NEFU_China--parts--CD1.png|600px|thumb|right|Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.]] |
| − | + | ||
| − | + | ||
<!-- --> | <!-- --> | ||
Revision as of 09:34, 20 October 2019
Cytosine deaminase
An enzyme that oxidizes 5-fluorouracil to 5-fluorouracil
Usage and Biology
result
Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil 5-FU, which has an extreme damage to the bacteria.
We added gradient of 5-FC culturing the bacteria and monitor their growth.
Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1140
- 1000COMPATIBLE WITH RFC[1000]