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This part includes the TVMV Protease cleavage site. TVMV cleaves the sequence ETVRFQS.
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The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.  
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We are using this recognition site to cut our protein in front of its polyG-tag so it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.
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We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.
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To read more about the sortase-mediated ligation, please have a look at our <a href=https://2019.igem.org/Team:TU_Darmstadt/Project/Sortase>wiki</a>.
 
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Revision as of 17:06, 21 October 2019


Tobacco Vein Mottling Virus Protease Cleavage Site

Profile

Name Tobacco Vein Mottling Virus (TVMV) Protease Cleavage Site
Base pairs 18
Origin Tobacco Vein Mottling Virus (TVMV)
Properties recognition site for the TVMV protease

Usage and Biology

The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.

We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.

To read more about the sortase-mediated ligation, please have a look at our wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The TVMV protease cuts after the final Gln. Cleavage works best if the following amino acid is Gly or Ser, but other amino acids at P1' except proline <a href="http://onlinelibrary.wiley.com/doi/full/10.1002/pro.506" target="_blank">work as well</a>