Difference between revisions of "Part:BBa K3165015"
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<h2> Usage </h2>
 
<h2> Usage </h2>
  
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We are using this promoter for the production of ccdB L83S mutant protein (<a Href ="https://parts.igem.org/Part:BBa_K3165014"> BBa_K3165014 </a>) by induction with arabinose.
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We are using this promoter for the production of ccdB L83S mutant protein <a href="https://parts.igem.org/Part:BBa_K3165014"> BBa_K3165014 </a> by induction with arabinose.
  
  

Revision as of 09:06, 20 October 2019


araBp

This part is used as an inducible promoter sensitive to varying concentrations of L-Arabinose. araBAD promoter is repressed by the presence of glucose and can be used to regulate gene expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

The araBAD promoter of the L-Arabinose operon in Escherichia coli allows tightly controlled, titrable expression of the gene of interest placed downstream the promoter. The promoter is sensitive to small changes in the concentration of arabinose while glucose serves as a repressor. The araBAD promotes has Pc subunit, O1 subunit, CAP binding subunit, I1 + I2 subunit and pBAD promoter region.

Usage

We are using this promoter for the production of ccdB L83S mutant protein <a href="https://parts.igem.org/Part:BBa_K3165014"> BBa_K3165014 </a> by induction with arabinose.


References

Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
Newman, J. R. & Fuqua, C. (1999). Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.