Difference between revisions of "Part:BBa K3196017"
Zyz2018hust (Talk | contribs) |
Zyz2018hust (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3196017 short</partinfo> | <partinfo>BBa_K3196017 short</partinfo> | ||
| − | FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. pelA can | + | FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. pelA can catalyze pectin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
Revision as of 06:51, 20 October 2019
AOX1-Kozak-FLO10 pro-pelA-His tag-AOX1 Terminator
FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. pelA can catalyze pectin.
Characterization
This is a four section for degrade and transfer pectin part.
DNA Gel Electrophoretic
After we link FLO10 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1986
Illegal BamHI site found at 937 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]