Difference between revisions of "Part:BBa K3254024"
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<partinfo>BBa_K3254024 short</partinfo> | <partinfo>BBa_K3254024 short</partinfo> | ||
| − | This is a superfold-GFP translational unit. The RiboJ can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence. | + | This is a superfold-GFP translational unit. The [[Part:BBa_K2615996|RiboJ]] can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence. |
=Usage= | =Usage= | ||
Latest revision as of 02:58, 21 October 2019
RiboJ-B0034-sfgfp
This is a superfold-GFP translational unit. The RiboJ can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence.
Usage
- This part can be used for quantitative testing the characteristics of transcriptional regulation device.
- The Ptac-sfGFP is a good example for such testing.
- You can see the page of BBa_K3254025 for getting more information.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 114