Difference between revisions of "Part:BBa K3179104"
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This part consists with promoter hEF1α and basic part rtTA3. hEF1α is a human elongation factor 1α gene promoter and is a strong mammalian promoter. hEFα is usually used in regular expression and has a stable expressional level, which is independent with cell type. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again. | This part consists with promoter hEF1α and basic part rtTA3. hEF1α is a human elongation factor 1α gene promoter and is a strong mammalian promoter. hEFα is usually used in regular expression and has a stable expressional level, which is independent with cell type. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again. | ||
| − | <br style="clear: both" /> | + | <br style="clear: both" />[[File:T-SYSU-CHINA-pCMEhEF1a_rtTA.jpg|500px|thumb|left]]<br style="clear: both" /> |
In our experiment, hEFα-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3. | In our experiment, hEFα-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3. | ||
Latest revision as of 04:33, 20 October 2019
hEF1a-rtTA3
hEF1α-rtTA3
Usage and Biology
This part consists with promoter hEF1α and basic part rtTA3. hEF1α is a human elongation factor 1α gene promoter and is a strong mammalian promoter. hEFα is usually used in regular expression and has a stable expressional level, which is independent with cell type. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.
In our experiment, hEFα-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 83
- 1000COMPATIBLE WITH RFC[1000]