Difference between revisions of "Part:BBa K112402:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K112402 short</partinfo> | <partinfo>BBa_K112402 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | ==pBca1256-K112402== | |
+ | <pre> | ||
+ | Biobricking of promoter for fabA protein | ||
+ | PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1) | ||
+ | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
+ | Product is pBca1256-K112402 | ||
+ | ---------------------------- | ||
+ | cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac | ||
+ | cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg | ||
+ | </pre> | ||
Latest revision as of 04:09, 24 October 2008
promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pBca1256-K112402
Biobricking of promoter for fabA protein PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 ---------------------------- cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg
Source
cloned from E. coli MG1655 genome