Difference between revisions of "Part:BBa K103006"
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<!-- Add more about the biology of this part here --> | <!-- Add more about the biology of this part here --> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | *OmpA is outer membrane protein from E. coli | + | *OmpA is outer membrane protein from E. coli [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] |
*Proteins fused to Ompa-link are presented on cell's surface | *Proteins fused to Ompa-link are presented on cell's surface | ||
*Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners | *Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners | ||
Revision as of 17:33, 24 October 2008
OmpA outer membrane protein A fused to linker; displays proteins on cell surface
One of our basic bricks used to create fusions attached to outer membrane
Usage and Biology
- OmpA is outer membrane protein from E. coli [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
- Proteins fused to Ompa-link are presented on cell's surface
- Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners
- In our project Ompa-link is used as outer membrane anchor for our selection system
- This brick contains our nonstandard restriction sites (NdeI and SacI) that allow 'scarless' cloning and easy creation of translation fusions
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]