Difference between revisions of "Part:BBa K103001:Experience"
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Revision as of 19:22, 21 October 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K103001
- Introduction of mutations to specific region of DNA.
User Reviews
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No review score entered. Keton |
We have tested this part in conjunction with T7 polymerase and target gene (lacZ) under T7 promoter in Top10 strain. Although we have obtained white colonies in various experimental setups sequencing revealed no mutations in target region (T7 promoter or lacZ ORF). We have repeated this experiment in GM2163 strain which is incapable of MutHLS mediated DNA repair due to lack of Dam and Dcm methyltransferases and obtained heritable white phenotype at high frequencies. Unfortunately we have no sequencing data to prove that mutations really occurred. |
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