Difference between revisions of "Part:BBa K3165040"
| Line 13: | Line 13: | ||
This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. | This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. | ||
| − | Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified | + | Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified. |
This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression. | This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression. | ||
Revision as of 01:29, 21 October 2019
ECFP under PcpcG2 expression
This part places the fluorescent protein - ECFP under the inducible pCpcG2 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified. This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.