Difference between revisions of "Part:BBa K3179002"
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− | MiRNA 885-5p-target is constructed bearing four repeats of miRNA 885-5p, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs. | + | MiRNA 885-5p-target is constructed bearing four repeats of miRNA 885-5p, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.<br style="clear: both" />This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to regulate the translation of mRNA with response parts. |
− | This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to regulate the translation of mRNA with response parts. | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 00:36, 20 October 2019
miR 885-5p-target
MiRNA 885-5p-target is constructed bearing four repeats of miRNA 885-5p, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to regulate the translation of mRNA with response parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]