Difference between revisions of "Part:BBa K2957015"

Latest revision as of 00:04, 20 October 2019

CMV, CCL5

Contents: CMV, CCL5, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1065
Illegal SpeI site found at 92
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 92
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1065
Illegal SpeI site found at 92
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1065
Illegal SpeI site found at 92
Illegal AgeI site found at 1071
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
-<partinfo>BBa_K2957099 short</partinfo>
+<partinfo>BBa_K2957015 short</partinfo>
 <b>Contents:</b> CMV, CCL5, SynthpA, with Carb resistance
@@ Line 16: / Line 16: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2957015 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K2957099 parameters</partinfo>
+<partinfo>BBa_K2957015 parameters</partinfo>
 <!-- -->