Difference between revisions of "Part:BBa K3165033"
(→‎Usage and Biology)
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Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker.  
 
Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker.  
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ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.
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ECFP paired with an RBS <html><a  href="https://parts.igem.org/Part:BBa_B0034">(BBa_B0034)</a></html> is used to generate a translational unit for the production of the fluorophore.
  
 
<h2> Usage </h2>
 
<h2> Usage </h2>

Revision as of 23:24, 20 October 2019


RBS + ECFP

The codon optimised engineered cyan fluorescent protein (ECFP) is placed along with a ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker. ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.

Usage

This part is used as a reporter unit under any suitable promoter. Under a photo-sensitive promoter system, this part can be used to characterise the functioning of the inducible system based on fluorescence measurements.