Difference between revisions of "Part:BBa K3105670:Design"

(→‎Design Notes)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
modified variant from natural GFP from <I>Aequorea victoria</I>
+
modified variant from natural GFP from <I>Aequorea victoria</I> (Zhang et al.)
  
 
===References===
 
===References===
 +
Zhang, Guohong, et al. “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells.” Biochemical and Biophysical Research Communications, 1996, doi:10.1006/bbrc.1996.1573.

Revision as of 23:46, 19 October 2019


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

eGFP was codon optimised for Pichia pastoris

Source

modified variant from natural GFP from Aequorea victoria (Zhang et al.)

References

Zhang, Guohong, et al. “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells.” Biochemical and Biophysical Research Communications, 1996, doi:10.1006/bbrc.1996.1573.