Difference between revisions of "Part:BBa K2957034"
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<b>Contents:</b> TRE promoter with C5a Gene fused to NeonGreen fluorescent protein, Carb resistance
 
<b>Contents:</b> TRE promoter with C5a Gene fused to NeonGreen fluorescent protein, Carb resistance
 +
<br>
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 
<b>What is it?:</b> A Composite part; we call it a pL1  
 +
<br>
 
<b>What does it do?:</b> It codes for the chemokine, C5a, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of neutrophil.  
 
<b>What does it do?:</b> It codes for the chemokine, C5a, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of neutrophil.  
 +
<br>
 
<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with HL-60s. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
 
<b>How to use it?:</b> This part was created with Type IIS Cloning and transfected into HEKs. We tested it with HL-60s. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
  

Revision as of 01:58, 20 October 2019


TRE, C5a-NeonGreen

Contents: TRE promoter with C5a Gene fused to NeonGreen fluorescent protein, Carb resistance
What is it?: A Composite part; we call it a pL1
What does it do?: It codes for the chemokine, C5a, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of neutrophil.
How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with HL-60s. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4128
    Illegal XbaI site found at 4175
    Illegal SpeI site found at 3240
    Illegal PstI site found at 621
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4128
    Illegal SpeI site found at 3240
    Illegal PstI site found at 621
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4128
    Illegal BglII site found at 2089
    Illegal BamHI site found at 637
    Illegal XhoI site found at 2205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4128
    Illegal XbaI site found at 4175
    Illegal SpeI site found at 3240
    Illegal PstI site found at 621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4128
    Illegal XbaI site found at 4175
    Illegal SpeI site found at 3240
    Illegal PstI site found at 621
    Illegal AgeI site found at 4181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3768
    Illegal SapI.rc site found at 3741