Revision as of 22:48, 19 October 2019 (view source) Satirev (Talk | contribs) ← Older edit		Revision as of 07:23, 20 October 2019 (view source) Zyz2018hust (Talk | contribs) Newer edit →
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	<partinfo>BBa_K3196024 short</partinfo>		<partinfo>BBa_K3196024 short</partinfo>
−	PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.	+	PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.
	<h1>'''Characterization'''</h1>		<h1>'''Characterization'''</h1>
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	<h1>'''DNA Gel Electrophoretic'''</h1>		<h1>'''DNA Gel Electrophoretic'''</h1>
−	After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.	+	After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.
	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]		[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]

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Revision as of 07:23, 20 October 2019

AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.

DNA Gel Electrophoretic

After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.

Sequence and Features