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<figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i></figcaption>
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<figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i>.</figcaption>
 
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Latest revision as of 22:42, 19 October 2019


L1c-PsaD-MHETase-3xHA-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the MHETase enzyme, kindly provided by the iGEM TU Kaiserslautern 2019 team, a 3xHA-tag, and the Rbcs2 terminator. The part can be used to express the MHETase enzyme in C. reinhardtii. The 3xHA tag can be used to detect and purify the MHETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
    Illegal NotI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2854
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
    Illegal NgoMIV site found at 1542
    Illegal NgoMIV site found at 2003
    Illegal NgoMIV site found at 2033
    Illegal NgoMIV site found at 2348
    Illegal NgoMIV site found at 2366
    Illegal NgoMIV site found at 2402
    Illegal NgoMIV site found at 3045
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E. coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii.