Difference between revisions of "Part:BBa K2984038"
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__NOTOC__
 
<partinfo>BBa_K2984038 short</partinfo>
 
<partinfo>BBa_K2984038 short</partinfo>
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L1c construct coding for the protein carbonic anhydrase.
 
  
  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This L1 part contains our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984079">carbonic anhydrase</a> sequence, a <a href="https://parts.igem.org/Part:BBa_K2984077">PsaD Promotor</a> and our <a href="https://parts.igem.org/Part:BBa_K2984076">Rbcs Terminator.</a>
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However we didn´t do any transformations in <i>chlamydomonas reinhardtii</i> or characterizations with this part because we had to prioritize other constructs.
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</span>
 
<partinfo>BBa_K2984038 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984038 SequenceAndFeatures</partinfo>
  

Revision as of 23:05, 21 October 2019


L1c-Psad-CBA-Rbcs2



This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This L1 part contains our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984079">carbonic anhydrase</a> sequence, a <a href="https://parts.igem.org/Part:BBa_K2984077">PsaD Promotor</a> and our <a href="https://parts.igem.org/Part:BBa_K2984076">Rbcs Terminator.</a>


However we didn´t do any transformations in chlamydomonas reinhardtii or characterizations with this part because we had to prioritize other constructs.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 2095
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]