Difference between revisions of "Part:BBa K2984033"

Latest revision as of 13:32, 7 January 2020

L1c-AR-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1c vector containing the arylsulfatase secretion peptide ARS1 and a mVenus fluorescent marker, flanked by AR promoter and RbcS2 terminator. The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in C. reinhardtii by fluorescence intensity measurements.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 953
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 268
Illegal PstI site found at 953
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 953
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 953
Illegal NgoMIV site found at 1430
1000
COMPATIBLE WITH RFC[1000]

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-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c vector containing the arylsulfatase secretion peptide <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS1</a> and a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> fluorescent marker, flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">RbcS2 terminator</a>.
-The construct is designed to test for the efficency of the secretion signal and the expression rates of the AR Promoter in <i>C.reinhardtii</i> with fluorescence intensity measurements.
+The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in <i>C. reinhardtii</i> by fluorescence intensity measurements.
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