Difference between revisions of "Part:BBa K3179101"

Latest revision as of 01:14, 20 October 2019

pTREL

This part consists of Vector pTRE, L7Ae, and miR592-target. There is a CMV promoter on pTRE that can transcribe mRNAs containing L7Ae and miR592-target. The translation of this mRNA is regulated by the amount of intracellular free miRNA592. When miRNA692 exists, the translation of this mRNA is suppressed. The mRNA gene product is L7Ae, which can combine with Kturn structure on mRNA to suppress the translation of that mRNA, acting as a regulatory part.

pTRE-L7Ae-miR592 is assembled into our circuit. When miRNA592 exists in the cell, the translation of L7Ae is suppressed and L7Ae cannot be produced,leading to the result that the mRNA with Kturn structure can be translated to express the response part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3179101 short</partinfo>
-This part is consisted of Vector pTRE, L7Ae and miR592-target. There is a CMV promoter on pTRE that can transcribe mRNAs with L7Ae and miR592-target. The translation of this mRNA is controlled by the amount of intracellular free miRNA592. When miRNA692 exists, the translation of this mRNA is suppressed. The mRNA gene product is L7Ae, which can combine with Kturn structure on mRNA to suppress the translation of that mRNA, acting as a regulatory part.
+This part consists of Vector pTRE, L7Ae, and miR592-target. There is a CMV promoter on pTRE that can transcribe mRNAs containing L7Ae and miR592-target. The translation of this mRNA is regulated by the amount of intracellular free miRNA592. When miRNA692 exists, the translation of this mRNA is suppressed. The mRNA gene product is L7Ae, which can combine with Kturn structure on mRNA to suppress the translation of that mRNA, acting as a regulatory part.
-pTRE-L7Ae-miR592 is assembled into our circuit. When miRNA592 exists in the cell, the translation of L7Ae is suppressed and L7Ae cannot be produced whereas the mRNA with Kturn structure can be translated to express the response part.
+pTRE-L7Ae-miR592 is assembled into our circuit. When miRNA592 exists in the cell, the translation of L7Ae is suppressed and L7Ae cannot be produced,leading to the result that the mRNA with Kturn structure can be translated to express the response part.