Difference between revisions of "Part:BBa K2924021:Design"

(→‎References)
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===Design Notes===
 
===Design Notes===
−
The acc genes were cloned in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
+
The genes from <i>E. Coli K12</> were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
  
  
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===Source===
 
===Source===
  
−
The genes were cloned from the genome of <i>E. coli</i>
+
The genes were cloned from the genome of <i>E. coli K12</i>

Revision as of 19:48, 20 October 2019


accABCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 668
    Illegal AgeI site found at 1501
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 803


Design Notes

The genes from E. Coli K12</> were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.


Source

The genes were cloned from the genome of <i>E. coli K12