Difference between revisions of "Part:BBa K2996008"
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蛋白胶图 | 蛋白胶图 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:04, 19 October 2019
pLuxR upstream of cas1/2
The expresssion of cas1-cas2 complex can be triggered by the addition of AHL.
- Usage and Biology
Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.
On the other hand, the input information is now acyl-homoserine lactones. In the transcription activation gene circuit, luxI can be put downstream of promotor J23001, enabling the expression of Luxl enzyme when dcas9 binds to lure sequence. AHL molecule production will then activate the expression of cas1-cas2 complex, in this way, the off-target incidence can be recorded.
- Result
蛋白胶图
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 258
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 979
- 1000COMPATIBLE WITH RFC[1000]