Difference between revisions of "Part:BBa K2957059"
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Contents: CMV | Contents: CMV | ||
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What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene. | What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene. | ||
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What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing. | What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing. | ||
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How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. | How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. | ||
Latest revision as of 21:44, 19 October 2019
CMV
Contents: CMV
What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene.
What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing.
How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 92
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 92
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 92
- 1000COMPATIBLE WITH RFC[1000]