Difference between revisions of "Part:BBa K3192026"

Revision as of 23:31, 21 October 2019

Double terminator designed for golden gate assembly

This terminator is a redesign of the double terminator BBa_B0015, a very well characterized iGEM terminator. BasI restriction sites were added to each terminator end to increase the ease of requesting this part for the intended use of golden gate assembly.

The 2019 Virginia iGEM team utilized Golden Gate Assembly for their project, and are aware it is a popular assembly method for many other iGEM teams. Through the addition of restriction sites compatible for Golden Gate, Virginia IGEM hoped to increase the ease of designing constructs for Golden Gate. Team planning on using this part and assembling using Golden Gate can now do so with more ease. Please see the design page for additional design notes on how to further optimize this BioBrick for Golden Gate Assembly.

File:T--Antiquity--Mfold-B0015-1.png

Pathway of styrene degradation into phenylacetic acid. Obtained from Substrate specificity and reaction mechanism of recombinant styrene oxide isomerase from Pseudomonas putida S12¹

Secondary Structure

File:Mfold-K3192026-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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 __NOTOC__
-{|width='80%'
-|-
-|width=35% valign='top'|
-[[Image:Terminator.png]]
-<partinfo>BBa_K3192026 parameters</partinfo>
-| width=50% valign='top' style='border: 1px solid black'|
 <partinfo>BBa_K3192026 short</partinfo>
 *
@@ Line 13: / Line 7: @@
 The 2019 Virginia iGEM team utilized Golden Gate Assembly for their project, and are aware it is a popular assembly method for many other iGEM teams. Through the addition of restriction sites compatible for Golden Gate, Virginia IGEM hoped to increase the ease of designing constructs for Golden Gate. Team planning on using this part and assembling using Golden Gate can now do so with more ease. Please see the design page for additional design notes on how to further optimize this BioBrick for Golden Gate Assembly.
-|}
+[[File:T--Antiquity--Mfold-B0015-1.png ‎|500px|thumb|left|Pathway of styrene degradation into phenylacetic acid. Obtained from Substrate specificity and reaction mechanism of recombinant styrene oxide isomerase from Pseudomonas putida S12<sup>1</sup>]]
 '''Secondary Structure'''