Difference between revisions of "Part:BBa K2984072"

 
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984072 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984072 SequenceAndFeatures</partinfo>
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===MoClo Overhangs===
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<p>This part with B5-B5 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984014">here</a>.</p>
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Latest revision as of 19:20, 19 October 2019


6x His-Tag for protein detection, isolation, purification

The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2+ or Co2+ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B5-B5 MoClo overhangs can be found here.