Difference between revisions of "Part:BBa K2997003"

Revision as of 19:13, 19 October 2019

Sigma 54-dependent transcriptional regulator (pchR)

The pchR gene can be translated into activator proteins that can bind with the inducer (p-Cresol) and leads to the conformation of the protein. In other words, the activator protein can bind with p-Cresol to form a protein-p-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-p-Cresol complex initiates the expression of the downstream gene, in our case GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1738
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1151
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1196
Illegal AgeI site found at 179
Illegal AgeI site found at 1079
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1105

Revision as of 19:12, 19 October 2019 (view source) Yeohcs88 (Talk \| contribs) ← Older edit		Revision as of 19:13, 19 October 2019 (view source) Yeohcs88 (Talk \| contribs) Newer edit →
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−	The pchR gene can be translated into activator proteins that can bind with the inducer (<i>p</i>-Cresol) and leads to the conformation of the protein. In other words, the activator protein can bind with <i>p</i>-Cresol to form a protein-<i>p</i>-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein - <i>p</i>-Cresol complex initiates the expression of the downstream gene, in our case GFP.	+	The pchR gene can be translated into activator proteins that can bind with the inducer (<i>p</i>-Cresol) and leads to the conformation of the protein. In other words, the activator protein can bind with <i>p</i>-Cresol to form a protein-<i>p</i>-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-<i>p</i>-Cresol complex initiates the expression of the downstream gene, in our case GFP.