Difference between revisions of "Part:BBa K3081024"
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This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases.
 
This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases.
  
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https://2019.igem.org/wiki/images/f/fb/T--Peking--CRISPRi_ssrA_ctrl_NT1.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:00, 21 October 2019


pBAD-dCas9-J23119-NT1-J23119-mRFP

This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases.



T--Peking--CRISPRi_ssrA_ctrl_NT1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
    Illegal NheI site found at 5590
    Illegal NheI site found at 5613
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 6173
    Illegal AgeI site found at 6285
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961