Difference between revisions of "Part:BBa K3113302"

Revision as of 18:49, 20 October 2019

pCAG_Gag-HiBiT-L7Ae

pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301^[1] and BBa_K3113203^[2]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3019
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2319

[1] ttps://parts.igem.org/Part:BBa_K3113301

[2] ttps://parts.igem.org/Part:BBa_K3113303

[1]

[2]

Revision as of 18:50, 19 October 2019 (view source) SarahiGEMMUC (Talk \| contribs) ← Older edit		Revision as of 18:49, 20 October 2019 (view source) Theresakeil (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3113302 short</partinfo>		<partinfo>BBa_K3113302 short</partinfo>

−	~~This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic~~ L7Ae~~. This~~ construct ~~forms virus-like particles which can be detected via a split luciferase assay. It~~ is ~~used~~ to ~~target FLuc mRNA specifically into virus~~-~~like particles via~~ L7Ae-C/~~Dbox~~ interaction.	+	pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301<ref>https://parts.igem.org/Part:BBa_K3113301</ref> and BBa_K3113203<ref>https://parts.igem.org/Part:BBa_K3113303</ref>).