Difference between revisions of "Part:BBa K3179001:Design"

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===Design Notes===
 
===Design Notes===
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L7Ae binds C/D boxes of sRNAs and guides RNA post-transcriptional modification; also binds KT-15 motif in 23S rRNA. L7Ae targets a specific sequence at the 5' end of the RNA, termed 2x-kturn. We use L7Ae to suppress another component 2xKturn-E1. Cooperating with another mRNA to play the role of miRNA sensor, identifying miRNA. We mutant one of its k-turn combine region, and reduce its cytotoxicity.
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This part is a mutation of previously part L7Ae. L7Ae is a switch of L7Ae-Kturn System. In this part, we mutant the 106th A to C and make the T to P after translation, which can lead to function losing of L7Ae because of the braking down of α-helix. In our design, this part is used to prove the suppress efficiency of L7Ae.
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===Source===
 
===Source===

Revision as of 18:22, 19 October 2019


L7AeO(a optimized L7Ae which is a transcriptional level repressor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a mutation of previously part L7Ae. L7Ae is a switch of L7Ae-Kturn System. In this part, we mutant the 106th A to C and make the T to P after translation, which can lead to function losing of L7Ae because of the braking down of α-helix. In our design, this part is used to prove the suppress efficiency of L7Ae.

Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References